Some Biophysical Properties of Virus Present in Tissue Cultures Infected with the Nuclear Polyhedrosis Virus of Trichoplusia ni
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چکیده
منابع مشابه
Characterization of DNA Polymerase Activity in Trichoplusia ni Cells Infected with Autographa californica Nuclear Polyhedrosis Virus
Nuclei isolated from Trichoplusia ni cells (TN-368) infected with Autographa californica nuclear polyhedrosis virus (AcMNPV) were used to study the replication of viral DNA. Based on DNA : DNA hybridization data, virus-specific DNA polymerase activity was insensitive to aphidicolin and novobiocin and was inhibited by ddTTP and N-ethylmaleimide. The data indicate that the AcMNPV-specific DNA pol...
متن کاملBaculovirus Replication: Stimulation of Thymidine Kinase and DNA Polymerase Activities in Spodopterafrugiperda Cells Infected with Trichoplusia ni Nuclear Polyhedrosis Virus
Baculoviruses are large DNA-containing viruses which replicate in the nucleus of invertebrate cells. The viruses contain supercoiled DNA of tool. wt. about 8 x l 0 7 (Bud & Kelly, 1977) and the multiply enveloped nuclear polyhedrosis virus of Trichoplusia ni used in this study has a genome size of 8.3 x 107 (Bud & Kelly, 1980). Little is known about the replication of baculoviruses in molecular...
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The protein solubilized from the proteinic crystalline structure surrounding the granulosis virus of Trichoplusia ni by use of a carbonate buffer (pH 10.7) gives a major component, as analyzed by ultracentrifugation, with a molecular weight of 180,000. This protein has heterogeneous subunit structure as demonstrated by estimates of molecular weights by use of gel electrophoresis, amino-, and ca...
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There have been several reports concerned with the replication and morphogenesis of insect baculoviruses during the past decade [1--7]. While there is general agreement as to the assembly of the virus on the basis of electron microscopic studies, there are still questions regarding the details of the replicative mechanisms, such as the acquisition of the virus envelope. Three possible ways have...
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in this thesis, at first we investigate the bounded inverse theorem on fuzzy normed linear spaces and study the set of all compact operators on these spaces. then we introduce the notions of fuzzy boundedness and investigate a new norm operators and the relationship between continuity and boundedness. and, we show that the space of all fuzzy bounded operators is complete. finally, we define...
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ژورنال
عنوان ژورنال: Journal of General Virology
سال: 1974
ISSN: 0022-1317,1465-2099
DOI: 10.1099/0022-1317-22-1-143